Effect of Nitrite on Anaerobic Metabolism of <i>Candidatus Accumulibacter Phosphates</i>

PENG Yong-zhen; GUO Chun-yan; XU Li-jie; LI Xi-yao; YUAN Zhi-guo

Journal of Beijing University of Technology > 2011 > 37(9): 1424-1429.

PENG Yong-zhen, GUO Chun-yan, XU Li-jie, LI Xi-yao, YUAN Zhi-guo. Effect of Nitrite on Anaerobic Metabolism of Candidatus Accumulibacter Phosphates[J]. Journal of Beijing University of Technology, 2011, 37(9): 1424-1429.

Citation:

PDF (418 KB)

Effect of Nitrite on Anaerobic Metabolism of Candidatus Accumulibacter Phosphates

1. Key Laboratory of Beijing Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China;
2. Advanced Wastewater Management Centre, the University of Queensland, Brisbane 4072, Australia

More Information

Received Date: June 09, 2009
Available Online: November 18, 2022

Graphical Abstract

Abstract

Abstract

In order to investigate the metabolism of Candidatus Accumulibacter Phosphates(a well-known phosphate accumulating organism) and its affecting factors,anaerobic-aerobic SBR reactor was performed to incubate polyphosphate accumulating organism(PAO) using synthetical sewage,and the content of PAOs reached more than 80%.Based on the enriched culture,several batch experiments were taken.The results indicated that when acetate and nitrite were both in the reactor(in 60min),with the increasing of the initial nitrite,Accumulibacter released much more PO₄^3--P,whereas the acetate uptake rate and the amount of accumulated PHA decreased.After 60min,the concentration of phosphate,nitrate and PHA were all degressive,suggesting that denitrifying phosphorus removal were occurred.The results suggested that there were denitrifying phosphorus organisms.Besides,there were a part of PAOs which could be poisoned by nitrite in this system.
- phosphate accumulating organism,
- nitrite,
- phosphorus release,
- denitrifying phosphorus removal

FullText(HTML)

References (16)

References

[1]	李捷,熊必永,张杰.电子受体对厌氧/好氧反应器聚磷菌吸磷的影响[J].哈尔滨工业大学学报,2005,37(5):619-622.LI Jie,XIONG Bi-yong,ZHANG Jie.Effects of different types of electron acceptors on phosphate uptake in anaerobic/aerobicprocess[J].Journal of Harbin Institute of Technology,2005,37(5):619-622.(in Chinese)
[2]	李相昆,周业剑,高美玲,等.亚硝酸根作为电子受体的反硝化吸磷特性[J].吉林大学学报:地球科学版,2008,38(1):117-120.LI Xiang-kun,ZHOU Ye-jian,GAO Mei-ling,et al.Nitrite serving as electronic acceptor for simultaneous denitrification andphosphorus removal[J].Journal of Jilin University:Earth Science Edition,2008,38(1):117-120.(in Chinese)
[3]	KUBA T,WACHTMEISTER A,VAN LOOSDRECHT M C M,et al.Effect of nitrate on phosphorus release in biologicalphosphorus removal systems[J].Wat Sci Tec,1994,30(6):263-269.
[4]	LU H,OEHMEN A,VIRDISA B,et al.Obtaining highly enriched eultures of candidatus accumulibacter phosphates throughalternating carbon sources[J].Wat Res,2006,40:3838-3848.
[5]	SMOLDERS G J F,VANDERMEIF J,VAN LOOSDRECHT M C M,et al.Model of the anaerobic metabolism of thebiological phosphorus removal process-stoichiometry and pH influence[J].Biotech Bioeng,1994,43(6):461-470.
[6]	国家环保局.水和废水检测分析方法[M].第3版.北京:中国环境科学出版社,1998.
[7]	BRAUNEGG G,SONNLEITNER B,LAFFERTY R M.A rapid gas chromatographic method for the determination of poly-β-hydroxybutyrate in microbial biomass[J].Eur J App Microbio,1978,6:29-37.
[8]	CHEN Y,CHEN Y S,XU Q,et al.Comparison between acclimated and unacclimated biomass affecting anaerobic-aerobictransformationsin the biological removal of phosphorus[J].Process Biochemistry,2005,40(2):723-732.
[9]	CROCETTI G R,HUGENHOLTZ P,BOND P L,et al.Identification of polyphosphate-accumulating organisms and design of16S rRNA directed probes for their detection and quantitation[J].Appl Environ Microbiol,2000,66(3):1175-1182.
[10]	刘建广,付昆明,杨义飞,等.不同电子受体对反硝化除磷菌缺氧吸磷的影响[J].环境科学,2007,28(7):1472-1476.LIU Jian-guang,FU Kun-ming,YANG Yi-fei,et al.Effect of electron acceptors on anoxic phosphorus uptake of DPB[J].Environmental Science,2007,28(7):1472-1476.(in Chinese)
[11]	WENTZEL M C,LOTTER L H,LOEWENTHAL R E,et al.Metabolic behaviour of Acinetobacter spp.in enhancedbiological phosphorus removal-a biochemical model[J].Water Sa,1986,12:209-224.
[12]	MINO T,VAN LOOSDRECHT M C M,HEIJNEN J J.Microbiology and biochemistry of the enhanced biological phosphateremoval process[J].Water Research,1998,32(11):3193-3207.
[13]	SUDIANA I M,MINO T,SATOH H,et al.Metabolism of enhanced biological phosphorus removal and non-enhancedbiological phosphorus removal sludge with acetate and glucose as carbon source[J].Water Science and Technology,1999,39(6):29-35.
[14]	COMEAU Y,HALL K J,HANCOCK R E W,et al.Biochemical-model for enhanced biological phosphorus removal[J].Water Research,1986,20(12):1511-1521.
[15]	SCHULER A J,JENKINS D.Enhanced biological phosphorus removal from wastewater by biomasswith different phosphoruscontents,partⅢ:anaerobic sources of reducing equivalents[J].Water Environment Research,2003,75(6):512-522.
[16]	PEREIRA H,LEMOS P C,REIS M A M,et al.Model for carbon metabolism in biological phosphorus removal processesbased on in vivo C-13-NMR labeling experiments[J].Water Research,1996,30(9):2128-2138.

[1]	ZENG Wei, LI Chao, MENG Qing'an, MA Chenyang. Efficiency of Denitrifying Phosphorus Removal and Population Dynamics of Candidatus Accumulibacter in MUCT[J]. Journal of Beijing University of Technology, 2019, 45(12): 1243-1251. DOI: 10.11936/bjutxb2018050003
[2]	PENG Yongzhen, PAN Cong, SUN Shihao, ZHAO Weihua, HUANG Yu. Effect of Influent ρ(C)/ρ(N) on the Nitrogen and Phosphorus Removal Performance of Pilot-scale AAO-BAF[J]. Journal of Beijing University of Technology, 2019, 45(9): 904-910. DOI: 10.11936/bjutxb2018030031
[3]	PENG Yongzhen, LÜ Dongmei, WANG Shuying. Effects of Feed Water COD and Different Adding Ways of COD on Denitrifying Phosphorus Removal of A ²O-BAF Process[J]. Journal of Beijing University of Technology, 2016, 42(9): 1406-1413. DOI: 10.11936/bjutxb2015090013
[4]	YE Lihong, LI Dong, ZHANG Jie, ZHANG Jinku, CENG Huiping. Advance of Research on the Technology of Nitrite-denitrifying Phosphorus Removal[J]. Journal of Beijing University of Technology, 2016, 42(4): 585-593. DOI: 10.11936/bjutxb2015070009
[5]	ZHANG Yongxiang, YAO Weitao, XIAO Sheming, REN Zhongyu, WANG Lei, LUO Xiaoya. Selection and Enrichment of Denitrifying Phosphate Removing Bacteria in Moving-bed Biofilm Reactor[J]. Journal of Beijing University of Technology, 2013, 39(1): 92-97.
[6]	LI Jun, ZHANG Yu-kun, WEI Su, WANG Ya-yi, MA Xiao, PENG Yong-zhen. Culture and Phosphorus Removal Property of Granular Sludge for Denitrifying Phosphorus Removal[J]. Journal of Beijing University of Technology, 2012, 38(3): 456-461.
[7]	PENG Yong-zhen, ZHANG Jing-qian, TANG Xu-guang, AKIO Takigawa. Study of the Characteristics of Denitrification Phosphorus Removal Using Nitrite as Electron Acceptor[J]. Journal of Beijing University of Technology, 2011, 37(11): 1749-1755.
[8]	ZENG Wei, LI Lei, YANG Ying-ying, ZHANG Yue, WANG Shu-ying. Study on Denitrifying Phosphorus Removal From Domestic Wastewater in A²O Process[J]. Journal of Beijing University of Technology, 2011, 37(9): 1407-1415.
[9]	RONG Hong-wei, PENG Yong-zhen, ZHANG Chao-sheng, ZHANG Hong, MA Yong. Effects of Nitrate on Denitrifying Phosphorus Removing and the Process Control[J]. Journal of Beijing University of Technology, 2009, 35(3): 385-390.
[10]	YANG Hong, ZHOU Qing-shui, LI Ruo-zheng, ZHANG Jie. Review of Removing and Recovering Phosphorus From Wastewater[J]. Journal of Beijing University of Technology, 2006, 32(10): 935-938,952.

Cited By

Get Citation

PDF

XML

Article views (15) PDF downloads (5)

Turn off MathJax

Article Contents

Abstract

References

Effect of Nitrite on Anaerobic Metabolism of Candidatus Accumulibacter Phosphates

Abstract

References

Related Articles

Catalog

Effect of Nitrite on Anaerobic Metabolism of Candidatus Accumulibacter Phosphates

Abstract

References

Related Articles

Catalog

Export File

Citation

Format

Content