Quantitative Analysis of Nitrifying Communities in Short-cut Nitrogen Removal From Municipal Wastewater of SBR

To investigate the effects of real-time control on the structure of nitrifying communities of the short-cut nitrogen removal in SBR pilot-scale plant with a working volume of 54 m³,molecular techniques of Fish,PCR-DGGE and PCR-Cloning-Sequencing were used to analyze ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) with the performance of short-cut nitrogen removal.Fish results show that in short-cut nitrogen removal process,AOB became dominant with compared to NOB and accounted for 3 % ~ 12 % of the total biomass.More importantly,no NOB was detected.PCR-DGGE gel results show that AOB were phylogenetically related to Nitrosomonas-like species with a melting point at the range between 30 % to 50 %.PCR-Cloning-Sequencing results show that all clones were affiliated with Nitrosomonas corresponding to DGGE results and 60%of the clones were affiliated with Nitrosomonas europaea.