Analysis of the Biofilm Microbial Community in the Three-stage Catching Bed Reactor Using PCR-DGGE and Cloning

ZHANG Yan; YAN Shengyong; ZHANG Yanqing; WANG Hui; YANG Zhengyang; WANG Lili

Journal of Beijing University of Technology > 2013 > 39(1): 122-130.

ZHANG Yan, YAN Shengyong, ZHANG Yanqing, WANG Hui, YANG Zhengyang, WANG Lili. Analysis of the Biofilm Microbial Community in the Three-stage Catching Bed Reactor Using PCR-DGGE and Cloning[J]. Journal of Beijing University of Technology, 2013, 39(1): 122-130.

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Analysis of the Biofilm Microbial Community in the Three-stage Catching Bed Reactor Using PCR-DGGE and Cloning

1. Key Laboratory of Beijing Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China;
2. Internal Trade Engineering Design and Research Institute, Beijing 100069, China

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Received Date: October 07, 2011
Available Online: January 10, 2023

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Abstract

The succession of the microbial community in the biofilm was studied in three-stage catching bed reactor system using PCR-DGGE and cloning technology.The homology of the microbial community in the biofilm was analyzed and the phylogenetic trees were constructed.Meanwhile,the contributions of microbial community in the biofilm on removal rates of COD and total nitrogen in the simultaneous nitrification denitrification(SND) system were discussed.The results showed that the microbial community in the biofilm appeared to have great evolution and high diversity during running.Furthermore,according to denaturing gradient gel electrophoresis(DGGE) analysis,the dominant bacterial community in the reactor could be mainly consisted of 5 groups: β-proteobacteria,γ-proteobacteria,unclassified bacteria,α-proteobacteria and Actinobacteria.In addition,β-proteobacteria had an advantage in numbers,which played an important role in the degradation of COD removal.Nitrosomonas sp.and uncultured Nitrospirae bacterium which could oxidize ammonia and nitrite in the system were also found,Pseudomonas.stutzeri which was recognized as aerobic denitrifying bacteria was detected in the reactor.
- simultaneous nitrification denitrification,
- PCR-DGGE,
- clone and sequence,
- biofilm,
- microbial community

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Analysis of the Biofilm Microbial Community in the Three-stage Catching Bed Reactor Using PCR-DGGE and Cloning

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