- 综合性科技类中文核心期刊
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- 中国科学引文数据库来源期刊
- 中国学术期刊文摘数据库（核心版）来源期刊
- 中国学术期刊综合评价数据库来源期刊
| Citation: |
ZENG Wei, LI Chao, MENG Qing'an, MA Chenyang. Efficiency of Denitrifying Phosphorus Removal and Population Dynamics of Candidatus Accumulibacter in MUCT[J]. Journal of Beijing University of Technology, 2019, 45(12): 1243-1251. DOI: 10.11936/bjutxb2018050003
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In this study, Modified University of Cape Town (MUCT) process was used to treat domestic sewage with low ρ(C)/ρ(N) ratio, and the relationship between population dynamics of dominant Candidatus Accumulibacter and efficiency of denitrifying phosphorus removal under nitration and nitrification was studied. Flow cytometry based quantification showed that Accumulibacter accounting for 22.1% of total bacteria were dominant phosphate accumulating organisms (PAOs) in system under nitrification. qPCR based estimation indicated that dominant species IIC, IIC(ppk1 excluding OTU NS D3) and IIF grew fast and accounted for 11.53%, 34.31% and 17.97% of Accumulibacter, respectively, while their ability using nitrite to achieve denitrifying phosphorus removal were stronger than that of other clades and were important for nitrite removal in system. As the most important clade in N and P removal in system, IID was always dominant along entire operation, which accounted for 80% of Accumulibacter under nitrification, but not as competitive as other Type Ⅱ clades under nitration.
| [1] |
LIU T K, CHEN P, CHEN H Y. Comprehensive assessment of coastal eutrophication in Taiwan and its implications for management strategy[J]. Mar Pollut Bull, 2015, 97(1/2):440-450. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=0159acf9c73298de0a7e1325ba1669e9
|
| [2] |
BARNARD J L. Biological nutrient removal without addition of chenicals[J]. Water Research, 1975, 9(5/6):485-490. https://www.researchgate.net/publication/229974133_Beckton_Demonstration_Biological_Nutrient-Removal_Plant
|
| [3] |
NAKAMURA K, HIRAISHI A, YOSHIMI Y, et al. Microlunatus phosphovorus gen-nov, sp-nov, a new gram-positive polyphosphate-accumulating bacterium isolated from activated-sludge[J]. International Journal of Systematic Bacteriology, 1995, 45(1):17-22. doi: 10.1099/00207713-45-1-17
|
| [4] |
HIEN T T N, VANG Q L, HANSEN A A, et al. High diversity and abundance of putative polyphosphate-accumulating Tetrasphaera-related bacteria in activated sludge systems[J]. Fems Microbiology Ecology, 2011, 76(2):256-267. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=1e7872bad9fe856cd4e28c3e824cae4f
|
| [5] |
SEVIOUR R J, KRAGELUND C, KONG Y, et al. Ecophysiology of the actinobacteria in activated sludge systems[J]. Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology, 2008, 94(1):21-33. doi: 10.1007/s10482-008-9226-2
|
| [6] |
COATS E R, WATKINS D L, BRINKMAN C K, et al. Effect of anaerobic HRT on biological phosphorus removal and the enrichment of phosphorus accumulating organisms[J]. Water Environment Research, 2011, 83(5):461-469. doi: 10.2175/106143010X12851009156402
|
| [7] |
MIELCZAREK A T, HIEN T T N, NIELSEN J L, et al. Population dynamics of bacteria involved in enhanced biological phosphorus removal in Danish wastewater treatment plants[J]. Water Research, 2013, 47(4):1529-1544. doi: 10.1016/j.watres.2012.12.003
|
| [8] |
HESSELMANN R, WERLEN C, HAHN D, et al. Enrichment, phylogenetic analysis and detection of a bacterium that performs enhanced biological phosphate removal in activated sludge[J]. Systematic and Appiled Microbiology, 1999, 22(3):454-465. doi: 10.1016/S0723-2020(99)80055-1
|
| [9] |
KEENE N A, REUSSER S R, SCARBOROUGH M J, et al. Pilot plant demonstration of stable and efficient high rate biological nutrient removal with low dissolved oxygen conditions[J]. Water Research, 2017, 121:72-85. doi: 10.1016/j.watres.2017.05.029
|
| [10] |
WELLES L, ABBAS B, SOROKIN D Y, et al. Metabolic response of "Candidatus Accumulibacter Phosphatis" Clade Ⅱ C to changes in influent P/C ratio[J]. Frontiers In Microbiology, 2017, 7:2121. http://www.ncbi.nlm.nih.gov/pubmed/28111570/
|
| [11] |
PETERSON S B, WARNECKE F, MADEJSKA J, et al. Environmental distribution and population biology of Candidatus Accumulibacter, a primary agent of biological phosphorus removal[J]. Environmental Mcirobiology, 2008, 10(10):2692-2703. doi: 10.1111/j.1462-2920.2008.01690.x
|
| [12] |
GEBREMARIAM S Y, BEUTEL M W, CHRISTIAN D, et al. Research advances and challenges in the microbiology of enhanced biological phosphorus removal-a critical review[J]. Water Environment Research, 2011, 83(3):195-219. doi: 10.2175/106143010X12780288628534
|
| [13] |
MAO Y, GRAHAM D W, TAMAKI H, et al. Dominant and novel clades of Candidatus Accumulibacter phosphatis in 18 globally distributed full-scale wastewater treatment plants[J]. Scientific Reports, 2015, 5:11857. doi: 10.1038/srep11857
|
| [14] |
国家环境保护总局.水和废水监测分析方法[M]. 4版.北京:中国环境科学出版社, 2002:38-47.
|
| [15] |
CROCETTI G R, HUGENHOLTZ P, BOND P L, et al. Identification of polyphosphate-accumulating organisms and design of 16S rRNA-directed probes for their detection and quantitation[J]. Applied and Environmental Microbiology, 2000, 66(3):1175-1182. doi: 10.1128/AEM.66.3.1175-1182.2000
|
| [16] |
FERRIS M J, MUYZER G, WARD D M. Denaturing gradient gel electrophoresis profiles of 16S rRNA-defined populations inhabiting a hot spring microbial mat community[J]. Applied and Environmental Microbiology, 1996, 62(2):340-346. http://d.old.wanfangdata.com.cn/OAPaper/oai_pubmedcentral.nih.gov_167804
|
| [17] |
HE S, GALL D L, MCMAHON K D. "Candidatus Accumulibacter" population structure in enhanced biological phosphorus removal sludges as revealed by polyphosphate kinase genes[J]. Applied and Environmental Microbiology, 2007, 73(18):5865-5874. doi: 10.1128/AEM.01207-07
|
| [18] |
ONG Y H, CHUA A S M, FUKASHIMA T, et al. High-temperature EBPR process:the performance, analysis of PAOs and GAOs and the fine-scale population study of Candidatus "Accumulibacter phosphatis"[J]. Water Research, 2014, 64:102-112. doi: 10.1016/j.watres.2014.06.038
|
| [19] |
GARCIA M H, IVANIVA N, KUNIN V, et al. Metagenomic analysis of two enhanced biological phosphorus removal (EBPR) sludge communities[J]. Nature Biotechnology, 2006, 24(10):1263-1269. doi: 10.1038/nbt1247
|
| [20] |
KLAPPENBACH J A, SAXMAN P R, COLE J R, et al. Rrndb:the ribosomal RNA operon copy number database[J]. Nucleic Acids Res, 2001, 29(1):181-184. doi: 10.1093/nar/29.1.181
|
| [21] |
GUIASAOLA A, QURIE M, DEL MAR VARGAS M, et al. Failure of an enriched nitrite-DPAO population to use nitrate as an electron acceptor[J]. Process Biochenistry, 2009, 44(7):689-695. doi: 10.1016/j.procbio.2009.02.017
|
| [22] |
LEAL C, AMARAL A L, COSTA M D L. Microbial-based evaluation of foaming events in full-scale wastewater treatment plants by microscopy survey and quantitative image analysis[J]. Environmental Science and Pollupion Research, 2016, 23(15):15638-15650. doi: 10.1007/s11356-016-6750-x
|
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