Abstract: Shear force is of significance for culturing stem cells on the biochip,which depends mainly on velocity gradient of the culture medium. Based on fluid dynamics,eight types of culturing areas were studied using finite element method and realizable κ-ε model in this paper. Distribution of velocity in culturing area and shear force in the center area were obtained. By comparing analysis results,one was selected from eight types of culturing areas according to the requirements of minimizing shear force and remaining less old medium,which were fitful for providing medium for stem cells on microfluidic chip. According to the results,two pieces of design experience were summed up.