Abstract: in this atricle, two intact species of microorganisms with specific and efficient activity have been cultured and separated for the biodegradation of Linear-Chain sodium dodecyl benzenesulfonate. Identification experiments are carried out in order to classify the two species biologically, which are identified as Alcaligenes sp.and Bacillus sp. Activity experiments with microorganisms are also conducted to substantiate the possibilily of their practical applications in water treatment engineering.