Abstract: Chironomid larvae occure in urban water supply systems, and their metabolite dissolutions containing biomass of amino acids, protein, fat and other organic matter have a higher potential to form the DBPs. In order to provide a theoretical basis for their impact on water quality and safety, we changed the different conditions like chlorine dosage, reaction time, pH and temperature to research the formation of disinfection by-products from the chlorination of chironomid larvae metabolite dissolution. The results showed that they reacted with chlorine to form chloral hydrate (CH) , 1, 1, 1-trichloroethane (1, 1, 1-TCA) 1, 1-dichloro-2-propanone (1, 1-DCP) and 1, 1, 1-trichloro-2-propanone (1, 1, 1-TCP) during disinfection. Higher chlorine dosage resulted in an increase of CH, 1, 1, 1-TCA and 1, 1, 1-TCP, but 1, 1-DCP concentration was increased at first and then decreased. Longer reaction time could accumulated CH, 1, 1, 1-TCA and 1, 1-DCP, but too long time could made 1, 1, 1-TCP decompose. Increasing pH was benefit to form chloral hydrate, 1, 1-DCP and 1, 1, 1-TCP, the maximum concentration of 1, 1, 1-TCA occurred at pH 7. And the four disinfection by-products concentrations were all increasing with temperature increasing.