Abstract: To solve the problem that it is difficult to determine the boundary condition when the temperature field of box girder is simulated in the process of temperature effect analysis for concrete box girder, a numerical calculation theory of meshless methods based on node modeling was adopted, a radial basis function collocation method model of concrete box girder was established based on the collocation method of meshlless method theory, and the steady-state temperature field inverse analysis of the cross-section of concrete box girder was studied. The research results show that the radial basis function collocation method can not only be used to conduct the numerical analysis of temperature field of concrete box girder, but also to calculate directly the temperature field of concrete box girder that is absent of boundary conditions, and there is high degree of consistency compared to calculated results of the finite element method.