Abstract: In order to keep the internal hollow structure strength of the printed 3D model and save printing materials, in this paper, the internal supporting structure of 3D model was studied by simulating the skeletal muscle structure. By watershed algorithm, one layer biological image was processed and combined with the target model on the printable layered structure. The 3D models was reproduced by matching cube method. Finally the needed model was output with the internal supporting structure through computation. By the analysis of the constitutive property with different models, it shows that with the method in this paper the strength of the model can be increased when the force of the specific direction appears. Also, when this method ensures the structural strength, it can save the printing material, and has better practical significance and theoretical research value too.