Abstract: To analyze the effects of parameters and working fluids on the power generation using LNG cold energy,this paper presents a series of simulation analysis of the basic operation data of the sub-critical Rankin cycle with several typical working fluids.The results show that there is an optimal evaporation temperature by testing output net power of the system,and different working fluids have different optimal evaporation temperature,which affects significantly output net power of the system.R290 is selected as optimal working fluid with net power of 81.34 kW in the optimal evaporation temperature of 11.08 ℃ conditions.The results can be the basis for further optimizing the cold energy power generation system.