Study on the Capacity and Induction of Rhodococcus sp-1 Biological Mn2+ Removal
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摘要: 利用传统的细菌分离方法,从地下水生物除锰滤池中分离、纯化出1株细菌,经LBB(leucoberbelin blue)法定性检测其具有Mn2+氧化能力,利用Sherlock细菌鉴定系统初步确定其属于红球菌属(Rhodococcus sp).对红球菌Rhodococcus sp-1菌株的生物除锰能力和诱导特性研究结果表明,Rhodococcus sp-1菌株在达到第1个生长稳定期,细菌浓度2.3×108个/mL时,其Mn2+的去除量可达到35 mg/L,具有很强的Mn2+生物去除能力;同时Fe的存在对Rhodococcus sp-1 Mn2+氧化活性的表达具有诱导作用,在相同培养条件下,Fe3+的诱导性强于Fe2+,其诱导速度是Fe2+的3~4倍;有机Fe3+的诱导性强于无机Fe3+,其诱导速度是无机Fe3+的1.6~2倍.Abstract: A strain bacteria had been separated,purified from the removal of manganese bio-filter treated with groundwater by traditional microbiology method.The strain bacteria could oxidate Mn2+ tested by the LBB method.It had been identified to belong to Rhodococcus sp.by the MIDI Sherlock bacteria identification system.Studied on the capacity and induction of Rhodococcus sp-1 biological manganese removal,the results shown: the removal amount of Mn2+ was 35mg/L when Rhodococcus sp-1 reached the first stabilization period,and it's order was 2.3×108n/mL.It had the strong capacity of biological Mn2+ removal.In addition Fe element could induct Rhodococcus sp-1 to oxidize Mn2+.Under the same conditions,Fe3+ was stronger than Fe2+,the induction speed ratio was 3~4∶1;organic Fe3+ was stronger than inorganic Fe3+,the induction speed ratio was 1.6~2∶1.
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Keywords:
- Rhodococcus sp. /
- biological Mn2+ removal /
- induction
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