应用BIAcore技术对KA-01及主要组分体外抗病毒研究
The Correspondence Study of BIAcore Binding and Anti-HBV Effect in Vitro by KA-01 and Its Main Ingredient
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摘要: 为了从中草药中找到抗HBV的有效药物,利用BIA技术分析比较中药复方KA-01及其组成部分A、B、C、D对HBsAg和HBeAg蛋白结合的作用,应用HepG2.2.15细胞模型,激光扫描共聚焦显微技术研究了KA-01及其主要组分体外抑制HBV蛋白分泌的活性.结果显示,KA-01、A、B、C和D与HBsAg蛋白结合的响应值分别为200、183.7、162.1、39.1、66.6 RU,与HBeAg蛋白结合很少.KA-01对HepG2.2.15细胞分泌HBsAg和HBeAg的抑制率分别为62.01%和30.59%,A对HepG2.2.15细胞分泌HBsAg和HBeAg的抑制率分别为54.26%和14.15%,B对HepG2.2.15细胞分泌HBsAg和HBeAg的抑制率分别为50.97%和27.92%,C对HepG2.2.15细胞分泌HBsAg和HBeAg的抑制率分别为17.54%和8.06%.KA-01,A,B体外具有抗HBV活性.Abstract: In order to discover activated ingredient form Chinese herbal medicine and to bind KA-01and its main ingredients to HBsAg and HBeAg protein in CM5 by BIAcore 3000,the HepG2.2.15 cell was cultured in vitro to observe the inhibitory effect of KA-01 and its main ingredient.The inhibitory effect of KA-01 and its main ingredients on protein secretion of HBV were tested by Laser Confocal Microscope.The results indicated that the value of KA-01and its main ingredients of A、B、C、D binding with HBsAg protein were 200、183.7、162.1、39.1、66.6 RU respectively,but almost 0 with HBeAg protein.The inhibitory effect of KA-01 on secretion of HBsAg and HBeAg were 62.01% and 30.59% respectively.The inhibitory effect of A on secretion of HBsAg and HBeAg were 54.26% and 14.15% respectively.The inhibitory effect of B on secretion of HBsAg and HBeAg were 50.97% and 27.92% respectively.The inhibitory effect of C on secretion of HBsAg and HBeAg were 17.54% and 8.06% respectively.The conclusion is that KA-01and its main ingredients of A、B have the inhibitory effect on HBV.
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