Abstract: In order to detect the overprint deviation in color printing, the article brought forward a method based on computer vision and established its algorithm. The image of the measuring mark of plate captured by CCD, recognized and analyzed the registered mark by the statistic character of hue in HSI space, and segmented the color image by the 2 paramaters of hue and intensity, measuring data about the registering quality can be obtained by hue projecting integral method after color image segmentation. Experimental results show that the system can fast enough measure the exact overprint deviation in color printing and have used to examine the overprint precision in Beiren Printing Machinery Co., Ltd.