Abstract: Based on the 10-mass model,the earthquake response of different natural period isolation structure are analyzed by using the equivalent linear method and time history analysis method according to the China, Japan and USA code in the paper.It is found that the response spectrum in long period part of design response spectrum of China code is too large,and the modified equation to the long period part is proposed based on the original acceleration response spectrum.In the end of the paper,aim at the shaking table test of a 5-story steel isolation structure,the earthquake response of isolation structure is compared by using the new suggestion modified acceleration response equation and time history analysis method,and the results obtained with the modified long period response spectrum is consist with the results of time history analysis and test.The response analysis results by using the suggestion equation of response spectrum value have a higher analysis precise for isolated structures.