霞草的组织培养

Tissue Culture of Gypsophila Elegans

摘要: 以霞草的不同部位为外植体均可诱导出愈伤组织,但只有部分愈伤组织能分化出芽。通过实验,得出了适合芽的分化的最佳培养基是MS+BA2(mg/L)+NAA0.5(mg/L)和MS+BAI(mg/L)+NAA0.1(mg/L),诱导生根的最佳培养基为1/2MS+NAA0.5(mg/L)和1/2MS+IBA0.5(mg/L).

Abstract: Regeneration potential of isolate organs of Gypsophila elegans was investigated. The callus was formed at the base of isolate shoots, apical buds and leaves. It was found that only some callus could produce bud. And tests show that the best mediums for budding are MS+BA2(mg/L)+NAA 0.5(mg/L) and MS+BAI(mg/L) NAA 0.1(mg/L), the best mediums for rooting are 1/2MS+NAA0.5(mg/L)and 1/ZMS+IBA0.5(mg/L).