Abstract: To solve the hydrogen peroxide(H₂O₂) damage of L₆ rat myoblast cells,adenosine triphosphate(ATP) solution of 1.210 48,2.420 96,and 4.841 92 g/L were used to treat myoblast cells,and MTT method,flow cytometry,DAPI fluorescence staining,HE staining and immunocytochemistry fluorescent apoptotic antibody were applied to test the damage.Results show that L₆ myoblast cells are damaged by H₂O₂,the cells survival rate significantly decreases and the apoptosis rate increases.The survival rate of L₆ myoblast cells increases with the concentrationes of each ATP solution,the expression of bcl-2 is promoted to increase and the expression of bax decreases to reduce the incidence of apoptosis.The degree of protection increases with decreasing ATP concentration.The effect is the most obvious in the dose of 1.210 48 g/L.The ATP solution has comparable protective effect of myoblast cells on H₂O₂-induced injury.The main mechanism of ATP solution is related to strengthen the expression of bcl-2 and inhibit that of bax through mTOR/STAT3 signal transduction with inhibiting apoptosis of cells.