干细胞培养微流控芯片培养区与剪切力有关的分析与设计

基金项目:

国家自然科学基金资助项目(50875007)

国家重大科学仪器专项(2011YQ030112)

详细信息

Analysis and Design on Culturing Area of Microfluidic Chip for Culturing Stem Cells Focusing on Shear Force

Institute of Laser Technology, Beijing University of Technology, Beijing 100124, China

摘要: 针对剪切力是芯片上影响干细胞培养的重要因素,其大小主要由培养基的流速梯度决定,利用realizable κ-ε有限元模型,对8种培养区结构进行了流体力学仿真,得出了培养区内的流速分布,并计算剪切力;通过对比分析,按照减小剪切力、减少液体残留2条要求,从8种结构中筛选出1种适合为干细胞培养提供培养液的结构.根据设计结果总结出2条设计经验.
- 干细胞 /
- 微流控 /
- 生物芯片 /
- 有限元法
Abstract: Shear force is of significance for culturing stem cells on the biochip,which depends mainly on velocity gradient of the culture medium. Based on fluid dynamics,eight types of culturing areas were studied using finite element method and realizable κ-ε model in this paper. Distribution of velocity in culturing area and shear force in the center area were obtained. By comparing analysis results,one was selected from eight types of culturing areas according to the requirements of minimizing shear force and remaining less old medium,which were fitful for providing medium for stem cells on microfluidic chip. According to the results,two pieces of design experience were summed up.
- stem cells /
- microfluidics /
- biochip /
- finite element method