Abstract: MnO₂ and Mn₃O₄ have been regarded as the contact oxidation catalyzer of Mn²⁺. The manganese in filtrate has not reached the drinking water standard. It has been proved that manganese removal is a process of biological catalysis oxidation. With the reproduction of biome with manganese removal bacteria as the nucleus, manganese removal efficiency increases. The population of manganese oxidizing bacteria is no less than n×10⁵~n×10⁶/mL. After sterilization mature and immature sands still have the capability of manganese removal, but after dipped in the solution of Mn²⁺ for 60 h, the capability disappeared. The transient manganese removal capability is the result of regeneration of adsorption capacity. The mechanism of biological fixation and removal of manganese is just based on these.