Abstract: In order to enhance the biodegradation efficiency of aniline wastewater at low temperature,we separated low-temperature aniline biodegraded bacteria JH-9 from wastewater treatment factory activated sludge with changing temperature domestication method.Biodegradation character and immobilization for JH- 9 were studied.The result shows that strain JH-9 classified as Acinetobacter-calcoaceticus.It can utilize aniline as the sole source of carbon and can endure 1 000 mg/L of aniline.After 52 h cultured the degradation rate of strain JH-9 can achieve 100% when provided with 150 mg/L aniline with low temperature at 7℃. We use Aspergillus Y3 as carrier.Mixed mycelial pellets forming by simultaneity incubated has less incubative time than which were asynchronously incubated.Besides,the number of immobilized bacteria,size,weigh and volume of mixed mycelial pellet are all bigger than that of mildew mycelail pellet.This is the first study reporting the new immobilization method-simultaneity incubated method.This method can solved the problem of conventional carrier,such as low efficiency mass transfer,less biomass and high cost,and will have great future in terms of application.Mixed mycelial pellets have high aniline biodegrading efficiency,after 66 h cultured the degradation rate of mixed mycelial pellets can achieve 100% when provided with 150 mg/L aniline with low temperature at 15℃.Strain JH-9 can retain activity after immobilization.