Abstract: An air-cathode microbial fuel cell (ACMFC) was constructed by using Pt/C as cathode,graphite felt as anode and glucose as the anode fed.The effects of fluid conditions and concentrations of glucose on electrochemical properties were studied.Resultsshow that the maximum power of approximately 85.9 mW/m² is obtained under the fluid's velocity 15 mL/min and density of glucose 1.0 g/L.The COD removal efficiency of ACMFC is 81.6% after running 48 h.