Fluorescence Measure and Control Flow Velocity for Micro-liquid in Micro-fluidic Chip PCR
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摘要: 为使微流控荧光PCR扩增循环的实际流速与理论设计流速吻合,保证芯片的PCR扩增过程与步骤的准确完成,必需对微通道中的实际工作流体流速情况进行实时测量和流速调控.利用在39个循环微通道的生物PCR微流控芯片中,充满强阳性荧光PCR试剂微通道的荧光信号值为空微通道的荧光信号值的4倍左右,为充满阴性荧光PCR试剂微通道荧光信号值的10倍左右的特性,在芯片上建立荧光实时测速信息反馈系统,对微通道中的实际工作流体流速情况进行实时反馈和控制.Abstract: In order to make the actual flow velocity of micro-channels be consistent with the theoretical value and make sure that the amplification process and the steps of PCR chip are completed exactly, it is needed to do real-time measurement and flow control of the actural work fluid flow condition.The fluorescence signal value of the micro-channel filled with the strong active fluorescence PCR reagent was about 4 times as great as that of the empty micro-channel and 10 times as great as that of the micro-channel filled with the negative fluorescence PCR reagent in the 39 cycled micro-channels of biological PCR micro-fluidic chip.Based on the above fact, a proposal was made that the real-time fluorescence detection micro-sensor should be set up on the chip to sense the information feedback, so the condition of the velocity of flow in the microchannel could be feed backed in time and the velocity of flow could be controlled.
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