Abstract: This paper focused on the sub-cellular distribution of thallium in several different fungus strains.The fungus strains were isolated from the Lanmuchang thallium-mineralised area in southwest Guizhou Province,and were used in the bio-concentration experiments.The sub-cellular supernatants including cytoplasmic,cellwall and cell organelles were seperated by the differential centrifuge after treated with snailase.Resultsshow that the bio-concentration rate of thallium in the nine strains decreases with increasing thallium contents in the culture,the maximum at 7 189 mg/kg and the maximum bio-concentration factor(BCF) at 7.19.In the sub-cellular supernatants detected by ICP-OES,the majority of thallium(up to 79.45%) was found in cytoplasmic organelles,and the concentrations of thallium in the order of priority are as follows: cytoplasmic-cellwall-cell organelles.Cytoplasmic supernatant is apparently a major storage site for thallium,which is a possible detoxification of thallium by the fungus.