Influence of P/C Ratios on the Dynamic Changes of the Organism Community for the Sequencing Batch Reactor (SBR) Enhanced Biological Phosphorus Removal System
-
摘要: 通过调节进水ρ（P）/ρ（C）的不同水平(2.9/100、1.4/100、0.57/100、0.29/100、1.4/100和2.9/100),考察了A/O-SBR系统强化生物除磷效果的动态变化;同时利用PCR-DGGE分子生物学技术,研究了聚磷菌和聚糖菌种群的竞争与演变.结果表明,当ρ（P）/ρ（C）逐渐降低时(2.9/100→1.4/100→0.57/100→0.29/100),吸收单位碳源的厌氧释磷量逐渐降低,而胞内糖原量逐渐升高.相应的DGGE图谱显示,微生物类群在ρ（P）/ρ（C）降低过程由11 OTUs升高到14 OTUs,最后降至7 OTUs;结合生化指标判断,系统优势菌种呈现的是从聚磷菌占优势、聚糖菌聚磷菌共存到聚糖菌占优势的动态变化.随后,提高进水ρ（P）/ρ（C）值从0.29/100到1.4/100再到2.9/100,污泥吸收单位碳源的厌氧释磷量逐渐升高,而胞内糖原量逐渐降低.这说明当聚糖菌占优势以后,通过调节ρ（P）/ρ（C）可重新培养获得聚磷菌优势系统,但DGGE图谱也显示,此时的聚磷菌优势种群较聚糖菌系统的优势种群已有较大变化,且与先前聚磷菌系统的优势种群也不尽相同.Abstract: The effect of the different P/C ratios,i.e.,2.9/100,1.4/100,0.57/100,0.29/100,1.4/100 and 2.9/100,on the dynamic changes of the phosphorus removal as well as the organism community was examined in an anaerobic/aerobic sequencing batch reactor(A/O-SBR) system,affiliated with Polymerase Chain Reaction-denaturing Gradient Gel Electrophoresis(PCR-DGGE).Results showed that when the P/C ratio dropped(2.9/100→1.4/100→0.57/100→0.29/100) gradually,the P-release to substrate-uptake ratios decreased companied by an increase in the intracellular glycogen content.Also,the DGGE profiles showed that with the decrease of the influent P/C ratios the dominant microbial species increased from 11 operation taxonomy units(OTUs) to 14 OTUs,and then dropped to 7 OTUs.Judged from the biochemical characteristics of the SBR,it can be confirmed that the predominant microbial populations in the SBR had changed from PAOs to PAOs and GAOs co-exist,and finally to GAOs.For the last recovery period,the P-release to substrate-uptake ratios increased gradually with the decrease of the intracellular glycogen content,indicating that PAOs can be re-cultivated through regulating the influent P/C ratio,once GAOs had dominated the EBPR system.While,the DGGE profiles showed that the dominant species of PAOs re-cultivated from the GAOs-dominated system was different greatly from both the GAOs-dominated system and the initial PAOs-dominated system.
-
-
[1] BROUGHTON A,PRATTS,SHILTON A.Enhanced biological phosphorus removal for high-strength wastewater with a lowrbCOD:P ratio[J].Bioresource Technology,2008,99(5):1236-1241.
[2] SEVIOUR R J,MINO T,ONUKI M.The microbiology of biological phosphorus removal in activated sludge systems[J].FEMS Microbiology Review,2003,27(1):99-127.
[3] OEHMEN A,ZENG R J,YUAN Z,et al.Anaerobic metabolism of propionate by polyphosphate-accumulating organisms inenhanced biological phosphorus removal systems[J].Biotechnology and Bioengineering,2005,91(1):43-53.
[4] OEHMEN A,LEMOS P C,CARVALHO G,et al.Advances in enhanced biological phosphorus removal:from micro to macroscale[J].Water Research,2007,41(11):2271-2300.
[5] 王亚宜,彭永臻,潘绵立,等.生物除磷系统聚糖菌的代谢机理及菌群结构[J].哈尔滨工业大学学报,2008,40(8):1319-1324.WANG Ya-yi,PENG Yong-zhen,PAN Mian-li,et al.Mechanism and the microbial community of glucose accumulationorganisms(GAOs)in enhanced biological phosphorus removal processes[J].Journal of Harbin Institute of Technology,2008,40(8):1319-1324.(in Chinese) [6] LIU W,NAKAMURA K,MATSUO T,et al.Internal energy-based competition between polyphosphate-and glycogen-accumulating bacteria in biological phosphorus removal reactors-effect of P/C feeding ratio[J].Water Research,1997,6(31):1430-1438.
[7] 王亚宜,鲁文敏,杨健,等.基于聚糖菌和聚磷菌竞争的代谢模型及影响因素[J].环境科学学报,2009,29(6):1131-1138.WANG Ya-yi,LUWen-min,YANG Jian,et al.Ametabolic model and impact factors based on competition between glycogenaccumulating organisms and phosphorus accumulating organisms in wastewater treatment[J].Acta Scientiae Circumstantiae,2009,29(6):1131-1138.(in Chinese) [8] LOPEZ-VAZQUEZ C M,OEHMEN A,HOOIJMANS C M,et al.Modeling the PAO-GAO competition:effects of carbonsource,pHand temperature[J].Water Research,2009,43(2):450-462.
[9] WANG Y,JIANG F,ZHANG Z,et al.The long-term effect of carbon source on the competition between polyphosphorusaccumulating organisms and glycogen accumulating organism in a continuous plug-flowanaerobic/aerobic(A/O)process[J].Bioresource Technology,2010,101(1):98-104.
[10] OEHMEN A,SAUNDERS A M,VIVES M T,et al.Competition between polyphosphate and glycogen accumulatingorganisms in enhanced biological phosphorus removal systems with acetate and propionate as carbon sources[J].Journal ofBiotechnology,2006,123(1):22-32.
[11] LOPEZ-VAZQUEZ C M,SONG Y,HOOIJMANS C M,et al.Temperature effects on the aerobic metabolism of glycogen-accumulating organisms[J].Biotechol Bioeng,2008,101(2):295-306.
[12] OEHMEN A,VIVES MT,LUH,et al.The effect of pHon the competition between polyphosphate-accumulating organismsand glycogen-accumulating organisms[J].Water Research,2005,39(15):3727-3737.
[13] SMOLDERS G J F,VAN DER MEIJ J,VAN LOOSDRECHT M C M,et al.Model of the anaerobic metabolism of thebiological phosphorus removal process:stoichiometry and pHinfluence[J].Biotech Bioeng,1994,43(6):461-470.
[14] 国家环境保护总局/水和废水监测分析方法委员会.水和废水监测分析方法[M].4版.北京:中国环境科学出版社,2002:243-285. [15] JENKINS D,RICHARD M G,DAIGGER G T.Manual on the causes and control of activated sludge bulking and foaming[M].2nd ed.New Jersey:Lewis Publishers,2003:79.
[16] BOSSHARD P P,GR TER D,STETTLER R,et al.Bacterial diversity and community composition in the chemocline of themeromictic alpine Lake Cadagno as revealed by 16S rDNA analysis[J].FEMS Microbiology Ecology,2000,31(2):173-182.
[17] MUYZER G,DE WAAL E C,UITTERLINDEN A G.Profiling of complex microbial populations by denaturing gradient gelelectrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA[J].Applied and EnvironmentalMicrobiology,1993,59(3):695-700.
[18] 苏俊峰,马放,王弘宇,等.利用PCR-DGGE技术分析生物陶粒硝化反应器中微生物群落动态[J].环境科学学报,2007,27(3):386-390.SU Jun-feng,MA Fang,WANG Hong-yu,et al.Application of PCR-DGGE to resolve microbial diversity in bio-ceramicreactor[J].Acta Scientiae Circumstantiae,2007,27(3):386-390.(in Chinese) [19] SAUNDERS A M,OEHMEN A,BLACKALL L L,et al.The effect of GAOs(glycogen accumulating organisms)onanaerobic carbon requirements in full-scale Australian EBPR(enhanced biological phosphorus removal)plants[J].WaterSci Technol,2003,47(11):37-43.
[20] PIJUAN M,SAUNDERS AM,GUISASOLAA,et al.Enhanced biological phosphorus removal in a sequencing batch reactorusing propionate as the sole carbon source[J].Biotechnol Bioeng,2004,85(1):56-67.
计量
- 文章访问数: 0
- HTML全文浏览量: 0
- PDF下载量: 0
下载:
