广西甜橙皮黄酮类等化学成分的研究

Study on the Constituents of the Flavonoids and Other Substances From the Peel of Citrus Sinensis (L.) Osbeck

摘要: 对广西甜橙皮中的黄酮类等化学成分进行了分离和鉴定。从中性乙醚提取物中分离得到5种结晶成分,经理化性质的测定及光谱分析,有3种物质鉴定为甲氧基取代的黄酮类化合物,分别为3,5,6,7,8,3',4',-七甲氧基黄酮(Ⅰ);蜜桔素(Ⅱ)和川陈皮素(Ⅲ),另2种物质分别鉴定为β-谷甾醇(Ⅳ)和木栓酮(Ⅴ).其中化合物Ⅰ和Ⅴ均是首次从国内柑桔果皮中分离得到,化合物Ⅰ的体外细胞抑瘤实验已初步表明,它对人体白血病细胞(K562)有很明显的抑制作用。

Abstract: Five compounds in the crystal form were isolated from dry peel of citrus Sinensis(L.)Osbeck collected from GuangXi province. By means of physico-chemical evidence andspectroscopic analysis(UV, IR, MS, ¹HNMR), three components were characterized as poly-methoxyflavonoids. They were 3, 5, 6, 7, 8, 3', 4'-heptamethoxy flavone (I), tangeretin (Ⅱ) andnobiletin (Ⅲ).β-sitosterol (IV) and friedelin(V), which were also identified. Among them, 3, 5, 6, 7, 8, 3', 4'-heptamethoxy flavone and friedelin were first obtained from domestic citrus peels. Heptamethoxy flavone was found having significant inhibitory action in the experiment with human Leukemic cell (K 562).