Abstract: A dynamic Driving simulation experiment of thirty subjects is carried out using the virtual driving simulator to analyze the variation of ECG and EEG indicators with driving time,and the experiment validates the effectiveness of the ECG and EEG indicators as the evaluation of driving fatigue.There is a significant correlation between EEG and ECG according to the Pearson Correlation.Finally,the relationship is established between EEG and ECG,and the overall index of driving fatigue is defined by Principal component analysis,which can exclude disturbed factors and lower data volatility,therefore,to improve accuracy of driving fatigue evaluation.