Abstract: The influence of the different operating conditions(the trans-membrane pressure,feed concentration and temperature) on dead-end MF specific resistance of yeast suspensions was studied by using a self-making microfiltration cup in which the PES flat membrane with 0.2μm average pore size was put.In addition,a linear multi-regression model was used to study the degree of the influence of these operating conditions on dead-end MF specific resistance of yeast suspensions.The results showed that,the trans-menbrone pressure, feed concentration and temperature are all influence factors of dead-end MF specific resistance,and the operating pressure is a remarkable factor,both the feed concentration and temperature are common influence factors;the influence degrees of the trans-membrane pressure,feed concentration and temperature are 75.31%,12.89%,11.8%,respectively;the specific resistance increases as the increasing trans-membrane pressure,increases with the increasing feed concentration first and then it decreases as concentration increases, whereas decreases as temperature increase;a relationship between specific resistance and the trans-membrane pressure,feed concentration and temperature is described by the linear multi-regression model.The average error between experimental value and model value is less than 5.16%.These will provide basis for process optimization in dead-end microfiltration.