Abstract: Agilent 86100C is the broadband sampling oscilloscope with a nominal bandwidth of 70 GHz.The pulse signal source with the transition duration of less than 2 ps is used to calibrate according to the tradtional calibration method.The national standard of the pulse parameter was established by NTN techonlogy and Agilent 86100B.The standard is not only a pulse signal source standard,but also an oscilloscope standard.However,the transition duration of pulse parameter national standard of China is(7.25±0.50) ps,and is not qulified for calibrating Agilent 86100C according to the traditional calibration method.The calibration method of Agilent 86100C using pulse parameter national standard is researched in this paper.The method extends the application of the pulse parameter national standard,and solves the calibration problem of Agilent 86100C at the same time.