Abstract: A stunning amount of informations is contained in results of human genome sequencing. Extracting, analyzing, and reading of the information have established a novel subject of bioinformatics in scientific frontier. Understanding the information, no doubt, will initiate a new age of speeding development for biology, medicine, pharmacy, and even mark an epoch in a global science. In this work, based on the "fail-safe" effect of organism, developing in a long-term evolution, we focus discussions on biological functions of non-coding DNA, which is over 98% of total human genome sequence. We analyse, in particular, the wobble, effect of codons-amino acids and codons-anticodons matrices on preventing genes from mutations during the process of DNA duplication or transcription. We clarify for the first time that, the reliability increased in the process of DNA transcription due to the existence of non-codin introns in a gene, and unveil that the global non-coding DNA, consisting mostly of transposon-derived repeats, constructs a frame-a carrier of genes, and palys a critical role in maintaining the structural stability of a DNA molecule, so that it may reduce, or even "shelter from" disturbances and injuries to genes in abnormal surroundings of a cell. All of those discussions provide many helpful references for preparing and designing anti-tumour medicamentos.