Abstract: To study the rationality and disaster prevention resilience of the spatial layout of urban epidemic prevention medical rescue system, a network model of a city's medical epidemic prevention and rescue was constructed by using complex network technology, analyzing its network topology and basic parameters of network characteristics, and studying the invulnerability of structures with edge and point connectivity as metrics under different attack models. By setting different network redundancy and network loads, the robustness of the network against deterministic attacks and random attacks were studied. The research result can provide theoretical support for the spatial optimization layout of urban epidemic prevention medical rescue system and the strengthening construction of core sites.