Abstract: To develop an effective warning strategy for driving fatigue on grassland highway, which is based on the prone points of driver fatigue during driving on grassland highway, drivers were divided into three types of stimulation preference (i.e., visual, auditory, and tactile) according to stress response theory and neuro-linguistic program theory. The existing typical anti-fatigue on-board equipment was referenced to determine the stimulation means and parameters for driving fatigue. A personalized anti-fatigue warning strategy was constructed to satisfy drivers' various preferences for stimulus selection. The effectiveness of this strategy was verified by driving simulator experiments. Results show that compared with other stimulus modes and blank control group, when the type of warning stimulus was preferred by drivers, the self-rating value of driving fatigue state, speed eigenvalue, acceleration eigenvalue, lateral offset eigenvalue and throttle power eigenvalue changed the least before and after the experiment. Drivers have the highest understanding and acceptance as well as the least disturbance of the preferred warning stimulus. The evaluation results by TOPSIS method indicated that different stimulus modes had different effects on alleviating driving fatigue. With the deepening of fatigue degree, drivers' preferred warning stimulus would alleviate driving fatigue more effectively.