Abstract: The nanochannels on the cell membrane of an organism are intelligently opened and closed in response to environmental stimuli. By precisely controlling the types of ions and the direction of ion flow, the material balance inside and outside the cell is maintained to keep normal life activities. Based on the concept of bionics, imitating biological nanochannels, artificial intelligence nanochannels with precise ion transport regulation functions was constructed, which shows broad application prospects in the fields of biosensing, nanotechnology, medical and energy. The research results of the our group in the field of biomimetic intelligent nanochannels in recent years were summarized, including the structure and function design of nanochannels, the construction and responsive ion transport behavior in response to external fields, and their applications in water-rich concentration cells. Furthermore, the application prospects of biomimetic intelligent nanochannels in important energy storage devices such as lithium secondary batteries, providing insight to develop high-performance ionic energy storage devices, were presented in this review.