Abstract: To improve the seismic performance of RC frame infilled wall, the RC frame with single row of steel bars shear wall was proposed and the seismic behavior of this structure was investigated.Two two-story, one-bay specimens were designed and fabricated. One was the RC frame with single row of steel bars shear wall, the other was RC frame infilled masonry wall. Based on the pseudo-static test, analysis was conducted on damage progression, failure mode, hysteresis property, skeleton curves, stiffness, energy dissipation capacity and ductility coefficient. Results indicate that there are two seismic fortification lines. Single row of steel bars shear wall is the first seismic fortification line and frame can function as the second resistant system. The design principle of strong pillar and weak beam, strong shear and weak bending, and strong node are achieved. It shows that the initial stiffness increases about 157% higher, the bearing capacity of the RC frame with single row of steel bars shear wall is improved about 134% higher than that of the RC frame infilled masonry wall, the bearing capability is stable in the later stage and the capacity of dissipating energy is preferable.