Abstract: To study the characteristics of lateral acceleration on the curve of small radius and the relationship between the lateral acceleration and lateral offset, simulation experiment of vehicle driving on curve section with small radius was carried out by driving simulator, and the lateral acceleration and lateral offset of the vehicle were obtained. Due to the small radius of the two-lane highway curved section by the lateral force, the characteristics of transverse acceleration on the curve segment were analyzed, and a mathematic model of vehicle lateral acceleration prediction and the correlation between lateral acceleration and lateral offset was established by using Matlab software. Results show that the lateral acceleration increases with the increase of the velocity, the lateral acceleration and lateral offset reach the maximum in the point of curve to spiral, and the lateral acceleration of circular curve segment has a logarithmic positive correlation with lateral offset. The results provide theoretical support for the safety analysis and accident prevention of two-lane road with small radius curve.