Abstract: To address the uncontrollability, unpredictability and non reproducibility of rat's behavior, an intelligent interactive control system was proposed. Different behaviors(stressful, neutral, friendly), which aims to stimulate a laboratory rat were generated by controlling a self-designed roboic rat with binocular vision. The response of a rat to a robotic rat was studied. The three-dimensional information of rats were effectively obtained based on binocular vision system. The experimental rat's behavioral characteristics was accurately analyzed by combining with an efficient image classification processing algorithm, and the behaviors of rats were modulated by controlling the behavior of the robotic rat, so as to achieve intelligent interaction. The social interaction experiments between the laboratory rats and the robotic rat show that the system is able to capture rat's behavioral characteristics in real time and direct rat's behavior by controlling the behavior of robotic rats. The system provides biologists, neurologists and brain scientists with a research platform and an assistant research tool.