Optimization of Extracellular Polymeric Substance Extraction Method of Different Sludge
-
摘要: 活性污泥胞外聚合物(extracellular polymeric substances,EPS)是污水生物处理过程中污泥结构、脱水性能、絮凝性能以及沉降性能的重要决定因素,而提取方法不同EPS的剥离程度存在很大的差别.因此EPS提取方法的建立至关重要.采用6种方法提取不同污泥EPS,分析了不同污泥每g干污泥中分层EPS的质量及组成,并考察了污泥粒径及脱水性能与污泥EPS的关系.结果表明:不同工艺不同污泥每g干污泥中EPS质量不同;甲醛+Na OH法提取EPS效率高且对细胞破坏程度小,试验中不同污泥EPS提取量为15.88~39.30 mg;每g干污泥中黏液层EPS质量越高,蛋白质与多糖质量浓度之比越大,污泥脱水性能越差;污泥EPS的提取效率与污泥粒径存在一定的相关性,污泥粒径越大,提取效率越高.
-
关键词:
- 胞外聚合物(EPS) /
- 提取方法 /
- 脱水性能 /
- 污泥粒径
Abstract: Extracellular polymeric substances( EPS) is an important determinant of the characters of sludge produced in biological wastewater treatment processes,including the structure,dewaterability,flocculation and settling behavior. However,the different extraction methods of EPS leaded to more discrepancy of EPS stripping degree. Thus,the establishment of the EPS extraction from sludge method is crucial. Six different methods were employed to extract EPS from different sludge in this study,and the content and composition of EPS in different sludge layers were analyzed. Meanwhile,the relationship between the sludge particle size and dewatering performance and the EPS were investigated.Resultsshow that the EPS content is different in different processes of reactor. Formaldehyde + Na OH extraction method was proved to be the most efficient method to extract EPS from sludge,and the destructiveness on cells of this method was relatively slight. The extracted EPS content of different sludge of the method is in the range of 15. 88- 39. 30 mg / g VSS. The content of slime-EPS is higher,the ratio of protein and polysaccharide is larger,but the dewaterability of the sludge is poorer. Besides,the sludge particle size is greater,and the extraction efficiency of EPS is higher. -
-
[1] NI B J,FANG F,XIE W M,et al.Characterization of extracellular polymeric substances produced by mixed microorganisms in activated sludge with gel-permeating chromatography,excitation-emission matrix fluorescence spectroscopy measurement and kinetic modeling[J].Water Research,2009,43(5):1350-1358.
[2] FLEMMING H C,WINGENDER J.The biofilm matrix[J].Nature Reviews Microbiology,2010,8(9):623-633.
[3] HOUGHTON J I,STEPHENSON T.Effect of influent organic content on digested sludge extracellular polymer content and dewaterability[J].Water Research,2002,36(14):3620-3628.
[4] D'ABZAC P,BORDAS F,VAN HUILEBUSCH E,et al.Extraction of extracellular polymeric substances(EPS)from anaerobic granular sludges:comparison of chemical and physical extraction protocols[J].Applied Microbiology and Biotechnology,2010,85(5):1589-1599.
[5] CAUDAN C,FILALI A,LEFEBVRE D,et al.Extracellular polymeric substances(EPS)from aerobic granular sludges:extraction,fractionation,and anionic properties[J].Applied Biochemistry and Biotechnology,2012,166(7):1685-1702.
[6] LI X Y,YANG S F.Influence of loosely bound extracellular polymeric substances(EPS)on the flocculation,sedimentation and dewaterability of activated sludge[J].Water Research,2007,41(5):1022-1030.
[7] SEVIOUR T,YUAN Z G,VAN LOOSDRECHT M C M,et al.Aerobic sludge granulation:a tale of two polysaccharides?[J].Water Research,2012,46(15):4803-4813.
[8] SHENG G P,YU H Q,LI X Y.Extracellular polymeric substances(EPS)of microbial aggregates in biological wastewater treatment systems:a review[J].Biotechnology Advances,2010,28(6):882-894.
[9] LIU H,FANG H H P.Extraction of extracellular polymeric substances(EPS)of sludges[J].Journal of Biotechnology,2002,95(3):249-256.
[10] NOVAK J T,SADLER M E,MURTHY S N.Mechanisms of floc destruction during anaerobic and aerobic digestion and the effect on conditioning and dewatering of biosolids[J].Water Research,2003,37(13):3136-3144.
[11] PELLICER-NACHER C,DOMINGO-FELEZ C,MUTLU A G,et al.Critical assessment of extracellular polymeric substances extraction methods from mixed culture biomass[J].Water Research,2013,47(15):5564-5574.
[12] YU G H,HE P J,SHAO L M,et al.Stratification structure of sludge flocs with implications to dewaterability[J].Environmental Science&Technology,2008,42(21):7944-7949.
[13] 倪丙杰,徐得潜,刘绍根.污泥性质的重要影响物质——胞外聚合物(EPS)[J].环境科学与技术,2006,29(3):108-110.NI B J,XU D Q,LIU S G.Important influence on the sludge properties of the material—extracellular polymeric substances(EPS)[J].Evnviron-mental Science&Technology,2006,29(3):108-110.(in Chinese) [14] TIAN Y,ZHENG L,SUN D Z.Functions and behaviors of activated sludge extracellular polymeric substances(EPS):a promising environmental interest[J].Journal of Environmental Sciences-China,2006,18(3):420-427.
[15] NGUYEN M T,MDHD YASIN N H,MIYAZAKI T,et al.Enhancement of sludge reduction and methane production by removing extracellular polymeric substances from waste activated sludge[J].Chemosphere,2014,117:552-558.
[16] WEI L L,WANG K,ZHAO Q L,et al.Fractional,biodegradable and spectral characteristics of extracted and fractionated sludge extracellular polymeric substances[J].Water Research,2012,46(14):4387-4396.
[17] LIANG Z W,LI W H,YANG S Y,et al.Extraction and structural characteristics of extracellular polymeric substances(EPS),pellets in autotrophic nitrifying biofilm and activated sludge[J].Chemosphere,2010,81(5):626-632.
[18] 钟方丽,曹宏斌,李鑫钢.交流电场对生物膜胞外聚合物提取过程的影响[J].中国环境科学,2003,23(3):243-246.ZHONG F L,CAO H B,LI X G.Effect of alternating electric field on the extraction of extracellular polymeric substances of biofilm[J].China Environmental Science,2003,23(3):243-246.(in Chinese) [19] 周俊,周立祥,黄焕忠.污泥胞外聚合物的提取方法及其对污泥脱水性能的影响[J].环境科学,2013,34(7):2752-2757.ZHOU J,ZHOU L X,HUANG H Z.Optimization of extracellular polymeric substance extraction method and its role in the dewaterability of sludge[J].Environmental Science,2013,34(7):2752-2757.(in Chinese) [20] BATSTONE D J,KELLER J,NEWELL R B,et al.Modelling anaerobic degradation of complex wastewater.Ⅰ:model development[J].Bioresource Technology,2000,75(1):67-74.
[21] ADAV S S,LEE D J.Extraction of extracellular polymeric substances from aerobic granule with compact interior structure[J].Journal of Hazardous Materials,2008,154(1/2/3):1120-1126.
[22] WANG Z P,LIU L L,YAO J,et al.Effects of extracellular polymeric substances on aerobic granulation in sequencing batch reactors[J].Chemosphere,2006,63(10):1728-1735.
[23] XIE B,GU J D,LU J.Surface properties of bacteria from activated sludge in relation to bioflocculation[J].Journal of Environmental Sciences-China,2010,22(12):1840-1845.
[24] NOVAK J T,MULLER C D,MURTHY S N.Floc structure and the role of cations[J].Water Science and Technology,2001,44(10):209-213.
[25] PAN X L,LIU J,ZHANG D Y,et al.A comparison of five extraction methods for extracellular polymeric substances(EPS)from biofilm by using threedimensional excitation-emission matrix(3DEEM)fluorescence spectroscopy[J].Water Sa,2010,36(1):111-116.
[26] DOMINIAK D M,NIELSEN J L,NIELSEN P H.Extracellular DNA is abundant and important for microcolony strength in mixed microbial biofilms[J].Environmental Microbiology,2011,13(3):710-721.
[27] YU G H,HE P J,SHAO L M.Novel insights into sludge dewaterability by fluorescence excitation-emission matrix combined with parallel factor analysis[J].Water Research,2010,44(3):797-806.
[28] ZHANG L L,FENG X X,ZHU N W,et al.Role of extracellular protein in the formation and stability of aerobic granules[J].Enzyme and Microbial Technology,2007,41(5):551-557.
计量
- 文章访问数: 0
- HTML全文浏览量: 0
- PDF下载量: 0
下载:
