Cultivation Process of AOB-Anammox Granular Sludge Under Micro-aerobic Condition
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摘要: 采用絮状硝化污泥和厌氧氨氧化颗粒污泥为接种污泥,利用膨胀颗粒污泥床反应器,在微氧曝气条件下,培养自养脱氮(氨氧化AOB-厌氧氨氧化Anammox)颗粒污泥.在无机高氨氮进水条件下,维持反应器运行58 d,成功培养出AOB-Anammox颗粒污泥.在模拟生活污水条件下,颗粒污泥脱氮效果稳定,氨氮和总氮去除率最高可达92.3%、71.2%,平均总氮去除负荷达1.237 kg·N/(m3·d).SEM及FISH结果表明:AOB-Anammox颗粒污泥微生物组成以2种菌群为主,AOB细菌密集排布于颗粒污泥表面,Anammox细菌均匀分布在颗粒污泥内部.Abstract: An expanded granular sludge bed(EGSB) reactor seeded with the mixture of flocculent nitrification sludge and anaerobic ammonium oxidation(Anammox) granular was started up to cultivate the ammonium oxidizing bacteria(AOB)-Anammox granular sludge under micro-aerobic condition.The mature AOB-Anammox granular sludge was successfully formed after performing of the EGSB reactor for58 days in terms of the high ammonia-nitrogen concentration and inorganic influent.Under the circumstance of synthetic domestic sewage,the performance of the sludge was testified and proven to be stable.The maximum removal rate of NH4+-N and total nitrogen(TN) reached 92.3% and 71.2% ,respectively,along with the average nitrogen loading removal(NLR) reaching 1.237 kg·N/(m3·d).Results of SEM and FISH analysis show that the AOB-Anammox granular sludge is mainly structured by two types of microflora.More specifically,AOB are configured on the surface of the sludge intensively while Anammox bacteria are homogeneously distributed inside the granular.
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