Abstract: Gender disguise is a common narrative form of opera and novel in Ming and Qing Dynasty. It is different from Western traditional sexology which refer gender disguise as psychosexual abnormal, the change of gender in gender disguise opera in Ming and Qing Dynasty is an independent identity practice, aims at reflecting the traditional deep-rooted cultural values and gender discipline. It is not only a reproduction of realistic gender character norms but also an arrogation and adjustment of stable gender narration. Gender disguise opera effectively promote the construction of dramatic environment, and gave birth to the artistic image with androgyny, which highlight the profound changes of the gender plays, deeply graved with cultural trace of specific historical period.