Abstract: This paper focuses on discussions about new ideas and mechanisms concerning the construction of the national science and technology innovation center from the points of high-end talents in Beijing, resources in science and technology innovation, the elements flow in science and technology innovation, and the interactive development in the program of "three cities and one district". At present, the program of the "three cities and one district" has made remarkable achievements, and its interactive development has entered the initial stage of exploration. However, there are still some problems, such as the needs to improve the coordinated mechanism, the overflow capacity of resources in scientific and technological innovation, and the smooth elements flow in innovation. The paper explores the path of elements flow in scientific and technological innovation in the program of "three cities and one district" from the aspects of talent, capital and technology, and the flow path of promoting its interactive development from the perspectives of government departments, enterprises and social organizations, in order to give full play to the role of the national science and technology innovation center by further releasing its innovation energy.